Yeah. The earlier, more famous posts of the blog on the article were about stuff that this book gives up on using because it just can't be contained and similar.
Then the blog goes into more and more dangerous stuff...
The silver lining is that the more dangerous a substance it, the lower the odds of it actually harming anybody, because everybody reacts like the author.
I know nothing from chemistry apart from high school stuff decades ago (and wasnt very good at it), but oh boy Derek knows how to write interesting stuff for laymen, read whole series since it gets quite regular mentions here.
This is how you make people interested in more difficult topics
My high school chemistry teacher used this approach, and I thank her for it. While maybe not at the same level of chemist, she did her best to make the subject much more approachable. My physics teacher had his humorous approach as well. I can still see his hand drawn sketches on the overhead in my mind.
This series by Derek Lowe is absolutely some of the funniest hard science writing I've ever had the pleasure of reading. That may sound like a low bar but make no mistake, this is truly great stuff. I've read the entire series of 33 articles going back over two decades and I only wish there was more (Derek is regular columnist but only occasionally posts new entries in this series).
I used to work in a genetics lab, and had to prepare trays of gel to visualise DNA tests (PCR testing to identify HLA alleles: effectively like finding someone's blood type, but for their immune system).
The compound in the gel that attaches itself to the DNA particles and makes it so we can visualise the results by UV fluorescence is Ethidium Bromide [1], one of the lower state Bromine salts the article mentions.
The way the electrophoresis gels are (were, by me and the other workers) prepared is by... microwaving a flask with it in. Repeatedly taking it out as it gets hotter to mix it.
Usually involving some release of steam. Which includes a small amount of the bromide. Fine in small doses, but when you do it multiple times a day, 5 days a week, 45 weeks of the year...
To this day I wonder how much of an effect it's going to have on me and my colleagues at the time.
This is why it's a known mutagen with a long list of very specific, strident toxicity warnings. That's the organic cation, though; compared to that, the Br- anion is utterly benign. You're looking at the wrong hazard!
Br- is a standard counterion in human-approved drugs (those formulated as HBr salts).
Bromide ion is in fact essential to animal (including human) life. It's necessary in trace amounts for the production of a weird S=N double bond in collagen IV, in a process where it is briefly converted to the BrO- ion (the +1 oxidation state). This was only discovered fairly recently; getting a fruit fly diet sufficiently depleted in bromide for the deficiency to show up was difficult.
Sea salt should have a fair amount of bromide in it. Bromine can be produced commercially by bubbling chlorine gas through sea water.
Apologies, I was writing this while balancing my son on one arm and mixed up some simplification with good old-fashioned mistakes!
I'll nip this in the bud and say "DNA particles" was definitely the wrong word.
You are correct, the Bromine here isn't really the problem; the fact that any intercalculation with DNA occurs is the main problem. Is it Bromine's fault? Not particularly, but it's an accessory in this instance. Based on anecdotal evidence it's common to the electrophoresis process.
My reason for bringing this up is just one of those relatable stories (/s) about why I also don't like working with Bromine (even if contextually it's not Bromine's fault, we love you Bromine!)
My PhD supervisor would wash his teeth over the sink where we'd rinse all the gel preparation tools.
He got throat cancer in his 50s and died soon after my PhD defense.
So, are you suggesting that the gel was the cause of the cancer for the supervisor? If so, was that sink’s drain connected to a diverter such that the waste didn’t enter the typical sewage system?
I don't want to hear any of these stories, considering I am a very clumsy person AND considering that I synthesized polycyclic aromatics. Thank god I got out after a couple of years, but sometimes I wonder what is still floating around in my DNA...
Yeah, that is always an option. However once boiling hot, if you introduce something into the flask it's more likely to create an explosion front.
A bit like when someone supercools a bottle of water and then taps it to make it freeze; except with exploding sticky gel, and it's as hot as lava*
*Not actually as hot as lava, but wow does it feel like it
I wouldn't worry about that. I've poured hundreds of gels and added the EtBr to it after microwaving it every single time. Never had anything close to that happen. Just use a sufficiently concentrated EtBr stock and you're adding like 45 µL to 45 mL.
> well, to the stuff described in this new paper., from Konrad Seppelt at the Freie Universität Berlin.
Prof. Konrad Seppelt became kind of famous in his community for creating various exotic Xenon substances including the world’s first compound between a noble gas and „noble“ metal.
He has been working with elemental fluorine for decades — so I guess he knows how to deal with dangerous stuff.
Xenon difluoride is in fact not hard to make: mix xenon and fluorine and expose to sunlight (or an artificial UV source). It just wasn't done for the longest time because of preconceptions.
I once remember sitting in a Physics class, they shared with Chemistry and as we're sitting there one of the chemistry teachers started clearing up - not sure what happened exactly but all of a sudden there's a bang, red smoke and everyone starts coughing - we had to evacuate the entire section.
Turns out the teacher had poured some bromine down a sink, along with something it reacted with.
My strongest memory of high school chemistry is inhaling bromine vapors (I'm sure we were doing various addition reaction common in organic chemistry). It's got a very specific smell, rarely if ever encountered in the regular world.
The same chemistry lab had a sign warning us not to "pipette by mouth", but I actually do that today to transfer tardigrades. It gives you remarkable control over the volume transferred.
That is a strong indictment of US teacher training. (One assumes that's the US, because teachers are remarkbly untrained over there.) Everyone knows you deactivate bromine and chlorine with thiosulfate or sulfite and only then chuck it down the drain.
This could also be the common error where someoone tries to pipet bromine like it was an ordinary liquid:
Open the bottle (under the hood), there's not that much red smoke coming out, dip the pipet into the dark liquid, pull in a few mLs, looks normal, almost like you could precisely dispense small amounts into the nearby target apparatus.
Lift the pipet slowly out of the source bottle, and carefully position it over the waiting reciever flask. Don't be too concerned about exact amounts to be dispensed any more, it can be seen that precision with bromine is not as ideal compared to something like pipetting alcohol, where you commonly lose a few drops anyway.
After all, immediately when you withdrew the pipet from the bromine bottle, the entire contents of the pipet had spewed out from its own gas pressure, into the inside of the hood. Don't worry about the state of your reaction, not a drop of bromine landed where you had intended.
All you can do if you have your wits about you is to hold your breath, carefully put the stopper back on the bromine bottle (without knocking the whole thing over) and fully evacuate, basically following out behind everyone else in other parts of the lab who knew there was a problem as soon as you did, just from the smell.
Let's be clear: all over the world people handle bromine safely by taking the proper precautions. The boiling point is low enough to squirt the material out of the pipette you picked it up with. Consequently, you exercise some patience and delicacy, wear gloves that are thick enough and operate in a fume hood.
If you can't do that you should be doing something else with your life. This isn't computing where nothing worse is at stake than money, usually.
I always enjoy Derek Lowe's "Things I won't work with", but I have to admit they're getting a little stale for me (although of course this was from 2019 and it wasn't stale then).
A writer can use the structure of their writing and word choices to hit certain emotional beats, and Lowe is quite good at this. If the actual content isn't quite substantial enough, though, the structural elements ring hollow to me because I've seen a lot of them.
It's directly analogous to clickbait titles, and also the subtle increase in head size, eye size, and emotional affect in YouTube thumbnails. The structure is used to encourage a level of emotional reaction in the consumer that the content of the title and/or thumbnail can't support in a more realistic style. But it starts to grate after a while.
As someone who have only A Level (i.e. high school) knowledge of Chemistry, every time I read "Things I won't work with" it always brings a smile to my face, as if those dangerous chemical are being synthesis in dodgy situation like those in cartoon.
There are a few things that have a pucker-factor beyond most sane peoples limit. A few people do die every year from accidents handling hazardous chemicals. Some have a bit of time to regret their mistakes, as they say goodbye to friends and colleagues.
Every clown that gets cavalier with safety rules should read the story of Karen Wetterhahn. I can't think of a worse accident except maybe Louis Slotin, as it reads like it is strait out of horror fiction.
Some scientists literally die for their work. Have a wonderful day =)
> Every clown that gets cavalier with safety rules should read the story of Karen Wetterhahn.
I don't understand what you are saying. Karen Wetterhahn meticulously observed all known precautions at the time. Her death was terrible, but as far as we know zero "clowning" was involved. Shouldn't someone who "gets cavalier with safety rules" study cases where someone gets hurt after they "gets cavalier with safety rules"? There is plenty of those.
The lesson of the Karen Wetterhahn incident is that "Do everything right and by the book? The gru can steal eat your face." Not really the thing clowns should focus on.
"Someone who followed every known rule perfectly still died horribly" should especially give the people who get cavalier about the known rules some pause.
I don't know. It doesn't do it for me. It makes me think "why bother with all this crap if. Even if you do them and you will die a sad and painful death".
There are much simpler, cleaner stories if you want to impart a message about the importance of PPE: Barry Weatherall was cleaning a pipe with sulphuric acid. He used PPE initially while performing the procedure, but removed it to do some paper work, and then went back to check on the progress without PPE. The acid exploded in his face and he become blind for life.[1]
It is a sad true story. Much cleaner than the Karen Wetterhahn one.
The message of the Karen Wetterhahn incident is "don't assume that just because you have PPE it is the right PPE for that task". While the message of the Barry Weatherall story is "wear your PPE".
The very first day I showed up for grad school, the entire hospital was closed off by men in hazmat suits and a bunch of firefighters. I proceeded to the central office of my program, where more men with hazmat suits were wandering around, and a guy was being treated in my program's office for injuries.
It turned out they were doing "spring cleaning" and somebody accidentally jiggled an old bottle of ether in a fume hood, and unfortunately the old ether must have had some free radicals, which led to an explosion. It blew out all the windows in the room (which were 20-30 feet away) and the chemist was only saved because he was wearing safety equipment (he still had permanent scars on his face years later).
Maybe in some far away galaxy, in a planet with much lower temperatures, intelligent life has evolved based on reactions like these, and some chemist there is writing about how long chains of carbon and hydrogen are for the most part useless as they're solid and inert at their temperature
That story is bonkers. I don't know how any student of chemistry mistakes a rocky crumbly cream-colored solid for an extremely hazardous red-orange liquid with a literal aura of evil wafting off of it.
Yep, that's it. A lot of links to other blog posts on older posts are broken, but you can usually change the "blogsdev" subdomain to "blogs" and it'll work.
Anhydrous redistilled hydrogen fluoride? No thanks!
Mind you: this is the stuff that, on skin contact, doesn't just burn you. It will immediately move through your skin to fuck up your nervous system, your organs, and your electrolyte balance to give you a heart attack.
If this synthesis were to kill me somehow, I'd rather blow myself up than get sprayed with HF.
Refer to [1] for more, unless you're easily disgusted...
> HF has actually been used right out of the cylinder for a long time in Merrifield peptide synthesizers. It's the traditional way to cleave the peptide off the resin at the final step, so there are actually a lot of people who've used the stuff. But it's in a dedicated apparatus that is (that had better be) well sealed, and people treat it with due respect. At a former employer of mine, there was an accident with one of these machines right before I joined the company. The shout "HF LEAK!" went out into the halls, and I'm told that the whole area set a never-to-be-equaled evacuation record.
Last night I somehow dreamed I had a hydrofluoric acid burn. Reading this thread I remembered there was this rust stain remover product on US Amazon that is 3% HF. https://www.amazon.com/product-reviews/B000LNQNM0/
It's got great reviews (and I'm inclined to believe that this product will do the job). There's one 1-star review that stands out though: "Simply put. It doesn’t work and it was shipped leaking." Yikes!
Who knows what was left of that 3% HF after it was shipped leaking... Maybe it was just mildly spicy water when delivered, and the poor amazon warehouse workers got to have most of the fun.
I’m sure they have all the equipment on hand to provide proper first aid for the vast multitude of chemicals they stock. They would never sell anything unsafe, right?
I thought surely you must be joking when I clicked on the link. Then I thought, ok, I'm a high school flunky, and I must be confusing this with the "don't go within a million miles of" substance that Walter White used to dissolve corpses and the one that I've read about repeatedly over the years that is immediately poisonous to human life even if you spill it only on a fingertip. But nope the 1-star reviews make it clear that I'm not confused. Then I start wondering how this can even be shipped with regular parcel services...
I finished up by thinking "he linked to the reviews, because the product is no longer for sale". So I click through the link, nope. $11/bottle. And not soem Engrishy label, looks like I could find it on the shelf at Home Depot or Walmart. Seriously, wtf.
Some fire suppression systems are not intended to save the lives of the people in the place where the fire is. E.g. in the ammunition store on a warship. They are intended to stop the fire before it triggers something much worse (like an explosion)
My dad had some interesting-acid stories, when he was a young chemist. They both involve cleaning glassware. The era is around 1970.
One was something he said resembled a wok full of the usual suspects of strong acids. Overnight, the mixture was hygroscopic, so they'd start it heating up in the morning to dehydrate it and increase the concentration. To test if it was ready, they'd throw in a small piece of wood, about the size of a match. If it disintegrated on contact, it was ready. Then you could dip your glassware to clean it.
But, in another lab, they would use a small bottle of hydrofluoric acid at the bench, taking a layer off the glass at the same time. While the dangers of large exposures were well known, he did mention the nerve damage and it being highly annoying and painful if trace amounts got under your fingernails, a somewhat common injury of the lab that everyone simply tolerated.
He thought the latter was a more convenient arrangement.
I'm a student in college studying cybersecurity but I have an interest in computer engineering.
I also have an interest in energetics. Florine is one of those chemicals you just got to respect. I know HF is used in the photoresist etch, and I'm interested in doing my own chip design. I realized that if I ever spilled HF on my, and the calcium cream didn't work, I'm better of just dying to anything other than HF. Iv heard its horrific to get on you in concentrated doses.
The price we pay for the small feature sizes on the wafer, and the increased density, in paid for in the usage of harsh chemicals.
Is there "Things I don't work with" series for programming? Alpha-quality compilers, non-ACID databases, legacy code full of race conditions, CSS (jk),...
PSA: try the Chemical Force Youtube channel. It is run by a crazy fellow who demonstrates all sorts of esoteric and highly exothermic inorganic chemistry.
I had read his post about Dengue a few days back, but hadn't looked at other posts, this guy is hilarious! The two articles about not enough nitro groups are great!
Kind of like alchemy. Except alchemy would kill you very slowly with heavy metal poisoning, and this stuff would kill you very very fast in multiple ways.
If you want non lethal, I remember Lowe had an article about something that wasn't explosive or poisonous... but it got complaints from the people in neighboring buildings because of the smell.
Edit: not sure this is the one I'm thinking of, because they were complaining about it in the neigboring village, but here's one:
> "Recently we found ourselves with an odour problem beyond our worst expectations. During early experiments, a stopper jumped from a bottle of residues, and, although replaced at once, resulted in an immediate complaint of nausea and sickness from colleagues working in a building two hundred yards away. Two of our chemists who had done no more than investigate the cracking of minute amounts of trithioacetone found themselves the object of hostile stares in a restaurant and suffered the humiliation of having a waitress spray the area around them with a deodorant. The odours defied the expected effects of dilution since workers in the laboratory did not find the odours intolerable ... and genuinely denied responsibility since they were working in closed systems. To convince them otherwise, they were dispersed with other observers around the laboratory, at distances up to a quarter of a mile, and one drop of either acetone gem-dithiol or the mother liquors from crude trithioacetone crystallisations were placed on a watch glass in a fume cupboard. The odour was detected downwind in seconds."
I have a specialization in Chemistry in my high school and we worked with Iodine and Bromide. IIRC Iodine is weak enough to be use as a desinfectant.
We used Bromide only oncde, probably disolved in carbon tetrachloride. A drop fell on my skin and it started to inch/hurt inmediately. I washed it imediately. It was not bad, just a whiter spot in the skin and some inching for a few days.
This is from Derek Lowe's wonderful "Things I won't work with" series from his long running blog.
Even if you're not a chemistry person, it's written in an accessible way, and usually quite humorous way.
I recommend his 2010 post on "FOOF" Dioxygen Difluoride from the same series.
[1] https://www.science.org/content/blog-post/things-i-won-t-wor...