I've still got my 3rd edition copy (from 1999 when I was an undergrad molecular biologist). Most of the basic biochemistry and molecular biology will be exactly the same--it hasn't changed much if at all. While there have been lots of additional details added over the last two decades, the fundamentals are unchanged for the most part.

This wouldn't apply to other fields such as Immunology (Janeway's Immunobiology) where I have purchased multiple copies of the years due to the field changing so fast.