We can't understand an organisms behavior in a hostile petri dish. Nothing lives on Mars, introducing an organism will produce results vastly different than anything we know on earth.
The only way we can control test this reliably is by building the worlds largest vacuated building, recreating the martian atmosphere below ~100ft (which may not be reliable as bacterial spores have been found well up in rain clouds), recreating the regolith to a compositional level, but also to a radiation level to account for all the free radical oxygens in it, then you have to recreate the light levels and daily cycles including UV and ionizing radiation. Then we'll have a reliable way to understand how the organisms will change the environment and how the environment may change the organisms.
We may be able to understand how the organisms will change the environment in the short term, but it's the long term that we're blind of and will remain blind of until we try. Introducing these organisms will change the environment, which will land new pressures on the organisms (IE evolutionary pressures) that could change the organisms meaning they'll deviate from our expected behaviors and our model for environment change will become increasingly inaccurate.
We can get a good bet through genome modelling (similar to what they did with the geobacter that can 'eat' iron, uranium, plutonium, etc), but in a high radiation environment we can only bet money on the radiotrophic fungus' and radioresistant bacterias to not mutate in ways we can't predict.
Perhaps the idea shouldn't be to recreate our environment, but to create a truly Martian one by giving it basic protobacteria and time.
The only way we can control test this reliably is by building the worlds largest vacuated building, recreating the martian atmosphere below ~100ft (which may not be reliable as bacterial spores have been found well up in rain clouds), recreating the regolith to a compositional level, but also to a radiation level to account for all the free radical oxygens in it, then you have to recreate the light levels and daily cycles including UV and ionizing radiation. Then we'll have a reliable way to understand how the organisms will change the environment and how the environment may change the organisms.
We may be able to understand how the organisms will change the environment in the short term, but it's the long term that we're blind of and will remain blind of until we try. Introducing these organisms will change the environment, which will land new pressures on the organisms (IE evolutionary pressures) that could change the organisms meaning they'll deviate from our expected behaviors and our model for environment change will become increasingly inaccurate.
We can get a good bet through genome modelling (similar to what they did with the geobacter that can 'eat' iron, uranium, plutonium, etc), but in a high radiation environment we can only bet money on the radiotrophic fungus' and radioresistant bacterias to not mutate in ways we can't predict.
Perhaps the idea shouldn't be to recreate our environment, but to create a truly Martian one by giving it basic protobacteria and time.